nbp2  (Novus Biologicals)

Journal: The Journal of Experimental Medicine

Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation

doi: 10.1084/jem.20252000

Figure Lengend Snippet: BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

Article Snippet: For the tracing studies, either AAV-hSyn-DIO-EGFP (cat #50457; Addgene), AAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (cat# 71760; Addgene), AAV-Ef1a-fDIO-EYFP (cat# 55641; Addgene), or AAV pEF1a-DIO-FLPo-WPRE-hGHpA (cat# 87306; Addgene) was utilized.

Techniques: Anterograde Tracing, Injection, Expressing, Saline, Control

Journal: Oncology Letters

Article Title: Proliferative escalation and possible neuroendocrine carcinoma transformation in pulmonary metastases of a cervical neuroendocrine tumor: A case report

doi: 10.3892/ol.2026.15501

Figure Lengend Snippet: Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) Synaptophysin, positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.

Article Snippet: The primary antibodies used for the immunostaining shown in and were as follows: synaptophysin (catalog no. 413831, clone 27G12; Nichirei Biosciences), chromogranin A (catalog no. M086901-2, clone DAK-A3; DAKO), p16 (catalog no. 550834, clone G175-405; BD Biosciences), YAP1 (catalog no. ab52771, clone EPR1674Y; Abcam), TTF-1 (catalog no. NCL-L-TTF-1, clone SPT24; NOVO), retinoblastoma protein (Rb; catalog no. 554136, clone G3-245; BD Biosciences), PAX8 (catalog no. ab53490, clone PAX8R1; abcam), and Ki-67 (catalog no. M7240, clone MIB-1; DAKO).

Techniques: Immunohistochemical staining, Staining, Expressing, Activity Assay

Journal: Oncology Letters

Article Title: Proliferative escalation and possible neuroendocrine carcinoma transformation in pulmonary metastases of a cervical neuroendocrine tumor: A case report

doi: 10.3892/ol.2026.15501

Figure Lengend Snippet: Histopathological and immunohistochemical findings of the left upper lobe lung tumor. (A) Low-power view of H&E staining (scale bar, 2.5 mm). (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100). (C) H&E staining (ocular, ×10; objective, ×20; final magnification, ×200). (A-C) The tumor consisted predominantly of NET components but with areas of increased chromatin and a higher nuclear-to-cytoplasmic ratio, suggesting the presence of NEC components. (D-K) Immunohistochemical analysis (ocular, ×10; objective, ×10; final magnification, ×100) revealed the following: (D) Synaptophysin, positive in NET areas but weaker in suspected NEC areas; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative in NET areas but focally positive in suspected NEC areas; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; and (K) Ki-67, high proliferative index in suspected NEC areas. NEC, neuroendocrine carcinoma; NET, neuroendocrine tumor.

Article Snippet: The primary antibodies used for the immunostaining shown in and were as follows: synaptophysin (catalog no. 413831, clone 27G12; Nichirei Biosciences), chromogranin A (catalog no. M086901-2, clone DAK-A3; DAKO), p16 (catalog no. 550834, clone G175-405; BD Biosciences), YAP1 (catalog no. ab52771, clone EPR1674Y; Abcam), TTF-1 (catalog no. NCL-L-TTF-1, clone SPT24; NOVO), retinoblastoma protein (Rb; catalog no. 554136, clone G3-245; BD Biosciences), PAX8 (catalog no. ab53490, clone PAX8R1; abcam), and Ki-67 (catalog no. M7240, clone MIB-1; DAKO).

Techniques: Immunohistochemical staining, Staining, Expressing